Available technologies

Institutes of First and Second Faculty of Medicine at Charles University in Prague

Sample Preparation and Laboratory Automation

• Covaris E220 Focused Ultrasonicator
• Tecan Freedom EVO® 150 Pipetting Robot

NGS Sequencing Technologies

• AVITI24 NextGen Multiomics Platform
• Illumina NovaSeq X
• Oxford Nanopore PromethION 2 Solo sequencer
• Oxford Nanopore MiniON long read sequencer
• ABI 3500xL Capillary Sequencers

NCMG Sequencing offers

Overview of available sequencing technologies (NovaSeq X Plus, AVITI24, and others), applications, and technical specifications can be found in the attached presentation – access is here:

PCR Technologies

• Bio-Rad CFX96 Touch Real-Time PCR System
• Applied Biosystems 7900HT Fast Real-Time PCR System
• Bio-Rad C1000 and T100 Thermal Cyclers

Supporting Infrastructure and Expertise

• Comprehensive equipment and expertise for sample preparation and bioinformatics data analysis
• Data storage and high-performance computing infrastructure
• Instrument specialists and bioinformaticians available for the infrastructure operation and data analysis

 

CEITEC MU Brno

• Massive paralel sequencers (Illumina MiSeq and Illumina NextSeq 500, MGI)
• Nanopore sequencing (Oxford Nanopore MinION a PromethION)
• Real-time qPCR and digital PCR (Life Technologies QuantStudio 12k, Life Technologies BioRad QX200, Roche LC480)
• System for single-cell analysis (10X Chromium)
• Automated pipetting and isolation Robot (TECAN FreedomEvo, Opentrons OT-2, RBC MagCore)
• Redudant data storage  and computational servers (cooperation  with eINFRA a ELIXIR-CZ)
• Flow cytometer and sorter (BD, cooperation with EATRIS-CZ)

Link for the list of equpiment: https://cfg.ceitec.cz/equipment/

Department of Medical Genetics, Faculty of Medicine in Pilsen, Charles University Prague and the University Hospital in Pilsen

• Opentrons OT-2 robotic liquid handler
• QIAGEN QIAxcel automated capillary electrophoresis instrument
• Massive parallel sequencers (Life Technologies IonTorrent, Illumina MiSeq)
• Capillary sequencers (Life Technologies)
• Real-time qPCR (Corbett Research, Qiagen)
• Invitrogen Qubit Flex fluorometer
• Fluorescent microscopes (Olympus)
• Data storage and computational servers
• DNA/RNA amplification systems

Biomedical centre of the Faculty of Medicine in Pilsen 

• Long-read sequencer PacBio Vega
• DNA microarray systems (Agilent, Innopsys)

Institute of Molecular and Translational Medicine

• Massively parallel sequencers (Illumina MiSeq and HiSeq Illumina, Qiagen PyroMark Q96, Roche GS Junior, Sequenom)
• Systems for automated preparation of sequencing libraries (Illumina Neoprep)
• Robotic system for nucleic acid isolation (Roche MagNA Pure 96 MagCore)
• Microarray system (Affymetrix)
• Real-Time qPCR (Roche LC 480, LC 1536, Cobas 4800, Eppendorf, BioRad CFX96)
• Data storage and computer servers
• The flow cytometer and sorter (BD FACS Aria Fusion II)
• Fluorescence microscope (Olympus BX60)
• Laser microdissection